Part:BBa_K3425037

TEV protease for Type IIS standard (RFC1000)

This part is protease from the Tobacco Etch Virus (TEV) which cleaves specific protein sequences such as BBa_K3425045. This enzyme is useful for regulated cleavage of various protein tags. The sequence is adapted to Type IIS iGEM standard assembly (RFC1000) and therefore lacks the start codon. When assembled using Type IIS standard, the ATG start codon is included in the scar between RBS and coding sequence part. You can find more information about how to use this standard in the Type IIS guide of Uppsala 2020 team.

The part is codon optimised for E. coli and B. subtilis and therefore the nucleotide sequence is distinct from TEV protease (BBa_K3102012) The protein sequence of this part (BBa_K3425037) is shorter than (BBa_K3102012).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 489
  Illegal NgoMIV site found at 795
  Illegal AgeI site found at 150
  
• 1000
  COMPATIBLE WITH RFC[1000]